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| Registros recuperados : 23 | |
| 4. |  | GALVÁN, G.; SOLLIER, S. Evaluación de cultivares de cebolla NRI-HRI 1993. In: INIA (Instituto Nacional de Investigación Agropecuaria); PROGRAMA HORTICULTURA. Resultados experimentales en cebolla 1993-1994. Reunión técnica. Canelones (UY): INIA Las Brujas, 1994. p. 44-47 (INIA Serie Actividades de Difusión ; 6)| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| 5. | | GALVÁN, G.; SOLLIER, S.; PACHECO, P.; ACOSTA, M.; CURBELO, N.; DOGLIOTTI, S. Crecimiento y rendimiento de poblaciones locales de morrón (Capsicum annuum L) bajo diferentes épocas de trasplante y criterios de cosecha (verde, rojo) ln: Congreso Nacional de Horticultura, 8.; Seminario Regional de Frutilla, 2001, Salto, Uruguay. Resúmenes. Montevideo (Uruguay): SUH; INIA, 2001. p. 27 "Sociedad Uruguaya de Horticultura; Instituto Nacional de Investigación Agropecuaria, Uruguay"| Biblioteca(s): INIA Las Brujas; INIA Salto Grande; INIA Tacuarembó; INIA Treinta y Tres. |
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| 8. | | Galván, G.Sollier, S.Ballestrino, L.Varga, C. Variabilidad intra poblacional de dos tipos locales de cebolla (Allium cepa L.) y de sus líneas endocriadas derivadas ln: Reunión Científica de Cebolla del Mercosur, 3., 1999, Salto, Uruguay Memorias. Montevideo (Uruguay): INIA, 1999. p. 94 Universidad de la República (Uruguay). Facultad de Agronomía; Instituto Nacional de Investigación Agropecuaria, Uruguay| Biblioteca(s): INIA Las Brujas. |
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| 9. | | Galván, G.Sollier, S.Záccari, F.Marquisa, S.Molla, C.Peluffo, S. Crecimiento, rendimiento y conservación poscosecha de cultivares de cebolla de diferente ciclo en diferentes localidades y fechas de siembra ln: Reunión Científica de Cebolla del Mercosur, 3., 1999, Salto, Uruguay Memorias. Montevideo (Uruguay): INIA, 1999. p. 93 Universidad de la República (Uruguay). Facultad de Agronomía; Instituto Nacional de Investigación Agropecuaria, Uruguay| Biblioteca(s): INIA Las Brujas. |
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| 13. | | Sollier, S.Galván, G.Acosta, M. Control de calidad en la producción de semilla de Pantanoso del Sauce CRS, nuevo cultivar nacional de cebolla ln: Galván, G.; Bugarín, G.; Vilaró, F., org. Trabajos presentados. Las Brujas, Canelones (Uruguay): INIA, 2001. p. 47-52 Proyecto de Validación de Tecnología PREDEG (MGAP)-BID| Biblioteca(s): INIA Las Brujas. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
19/06/2019 |
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Actualizado : |
05/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
ALMEIDA, D.S.; PAZ, L.N.; DE OLIVEIRA, D.S.; RISTOW, P.; HAMOND, C.; COSTA, F.; PORTELA, R.W.; ESTRELA-LIMA, A.; PINNA, M.H. |
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Afiliación : |
Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil.; Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil.; Instituto Gonçalo Moniz, Ministério da Saúde, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil Silva, D.N. // Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil.; Laboratory of Bacteriology and Health, Institute of Biology, Federal University of Bahia, Salvador, Bahia, Brazil.; CAMILA HAMOND, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Saude Coletiva, Federal University of Bahia, Salvador, Brazil.; Instituto de Ciências da Saúde, Federal University of Bahia, Salvador, Brazil.; Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil.; Post Graduate Program in Animal Science in the Tropics-Federal University of Bahia., Salvador, Bahia, Brazil. |
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Título : |
Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
PloS One, 2019, Volume 14, Issue 5, Page e0217391. [OPEN ACCESS.].Doi: https://doi.org/10.1371/journal.pone.0217391 |
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DOI : |
10.1371/journal.pone.0217391 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: August 14, 2018 / Accepted: May 11, 2019 / Published: May 23, 2019. This study was funded by the Foundation
for Research Support of the State of Bahia, Brazil (http://www.fapesb.ba.gov.br/), Public Notice no.025/2010 under Term of Grant PPP 0034/2011,coordinated by Melissa Hanzen Pinna. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
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Contenido : |
ABSTRACT.
The study aimed to evaluate the histopathological characteristics of renal lesions in chronically infected sheep and with low titers of anti-Leptospira antibodies from a slaughterhouse. In the serological analysis, 24.74% (48/194) presented seroreactivity with a titer equal to or greater than 100. Among these seroreactive sheep, titers of 100 were predominant (58.33%, 28/48), with the highest titer being 1,600 (2.08%, 1/48). Serogroup Sejroe (sv. Hardjo) was the most frequent at 35.42% (17/48). Leptospiral DNA was verified in 4.12% (8/194) of the kidney samples tested, and no urine sample was positive. All the samples corresponded to the pathogenic species L. interrogans. The eight amplicons with 202-nucleotides were identical with two mismatches (presented 100% of identity) using the PCR targeting to secY gene. Histological sections of PCR-positive kidneys were submitted to direct detection by the anti-LipL32 immunohistochemistry (IHC) technique. The Leptospira spp. antigen was evident in 62.5% (5/8) of the kidneys. Positive staining was observed in the cytoplasm of tubular cells and in the form of brownish aggregates that adhered to tubular epithelial cells and projected into the lumen. Inflammatory lymphoplasmacytic infiltrate, ranging from mild to moderate, with multifocal distribution, was the predominant finding in seroreactive animals (33.33%, 16/48). The demonstration of the leptospiral antigen lining the renal tubules through IHC of naturally infected sheep confirmed by PCR characterizes renal colonization in a species with the presence of histological changes compatible with leptospirosis. MenosABSTRACT.
The study aimed to evaluate the histopathological characteristics of renal lesions in chronically infected sheep and with low titers of anti-Leptospira antibodies from a slaughterhouse. In the serological analysis, 24.74% (48/194) presented seroreactivity with a titer equal to or greater than 100. Among these seroreactive sheep, titers of 100 were predominant (58.33%, 28/48), with the highest titer being 1,600 (2.08%, 1/48). Serogroup Sejroe (sv. Hardjo) was the most frequent at 35.42% (17/48). Leptospiral DNA was verified in 4.12% (8/194) of the kidney samples tested, and no urine sample was positive. All the samples corresponded to the pathogenic species L. interrogans. The eight amplicons with 202-nucleotides were identical with two mismatches (presented 100% of identity) using the PCR targeting to secY gene. Histological sections of PCR-positive kidneys were submitted to direct detection by the anti-LipL32 immunohistochemistry (IHC) technique. The Leptospira spp. antigen was evident in 62.5% (5/8) of the kidneys. Positive staining was observed in the cytoplasm of tubular cells and in the form of brownish aggregates that adhered to tubular epithelial cells and projected into the lumen. Inflammatory lymphoplasmacytic infiltrate, ranging from mild to moderate, with multifocal distribution, was the predominant finding in seroreactive animals (33.33%, 16/48). The demonstration of the leptospiral antigen lining the renal tubules through IHC of naturally infected shee... Presentar Todo |
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Palabras claves : |
DISEASES OF SHEEP; LEPTOSPIRES; PLATAFORMA EN SALUD ANIMAL. |
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Thesagro : |
ENFERMEDADES DE LOS ANIMALES; LEPTOSPIROSIS. |
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Asunto categoría : |
E16 Enfermedades de los animales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16705/1/pone.0217391.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6532964/pdf/pone.0217391.pdf
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Marc : |
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100 1 $aALMEIDA, D.S.
245 $aInvestigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse.$h[electronic resource]
260 $c2019
500 $aArticle history: Received: August 14, 2018 / Accepted: May 11, 2019 / Published: May 23, 2019. This study was funded by the Foundation for Research Support of the State of Bahia, Brazil (http://www.fapesb.ba.gov.br/), Public Notice no.025/2010 under Term of Grant PPP 0034/2011,coordinated by Melissa Hanzen Pinna. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
520 $aABSTRACT. The study aimed to evaluate the histopathological characteristics of renal lesions in chronically infected sheep and with low titers of anti-Leptospira antibodies from a slaughterhouse. In the serological analysis, 24.74% (48/194) presented seroreactivity with a titer equal to or greater than 100. Among these seroreactive sheep, titers of 100 were predominant (58.33%, 28/48), with the highest titer being 1,600 (2.08%, 1/48). Serogroup Sejroe (sv. Hardjo) was the most frequent at 35.42% (17/48). Leptospiral DNA was verified in 4.12% (8/194) of the kidney samples tested, and no urine sample was positive. All the samples corresponded to the pathogenic species L. interrogans. The eight amplicons with 202-nucleotides were identical with two mismatches (presented 100% of identity) using the PCR targeting to secY gene. Histological sections of PCR-positive kidneys were submitted to direct detection by the anti-LipL32 immunohistochemistry (IHC) technique. The Leptospira spp. antigen was evident in 62.5% (5/8) of the kidneys. Positive staining was observed in the cytoplasm of tubular cells and in the form of brownish aggregates that adhered to tubular epithelial cells and projected into the lumen. Inflammatory lymphoplasmacytic infiltrate, ranging from mild to moderate, with multifocal distribution, was the predominant finding in seroreactive animals (33.33%, 16/48). The demonstration of the leptospiral antigen lining the renal tubules through IHC of naturally infected sheep confirmed by PCR characterizes renal colonization in a species with the presence of histological changes compatible with leptospirosis.
650 $aENFERMEDADES DE LOS ANIMALES
650 $aLEPTOSPIROSIS
653 $aDISEASES OF SHEEP
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700 1 $aRISTOW, P.
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700 1 $aCOSTA, F.
700 1 $aPORTELA, R.W.
700 1 $aESTRELA-LIMA, A.
700 1 $aPINNA, M.H.
773 $tPloS One, 2019, Volume 14, Issue 5, Page e0217391. [OPEN ACCESS.].Doi: https://doi.org/10.1371/journal.pone.0217391
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